Myeloperoxidase (Mpo) By Flow Cytometry

Myeloperoxidase (MPO) by Flow Cytometry

What it is

The Myeloperoxidase (MPO) by Flow Cytometry test measures the presence and expression of the MPO enzyme within white blood cells, particularly myeloid cells. Myeloperoxidase is an important marker for myeloid lineage differentiation and is commonly used in the diagnosis and classification of acute leukemias. Flow cytometry provides rapid, sensitive, and quantitative detection of MPO at the single-cell level.

Uses

This test is primarily used in hematology to help distinguish acute myeloid leukemia (AML) from acute lymphoblastic leukemia (ALL). It is also used to evaluate myeloid lineage in cases of undifferentiated or ambiguous leukemias. The test supports clinicians in making accurate diagnoses and guiding appropriate treatment strategies.

Symptoms That May Lead to the Test

Doctors may order this test if you present with symptoms suggestive of leukemia or bone marrow disorders, such as persistent fatigue, frequent infections, easy bruising or bleeding, unexplained fever, enlarged lymph nodes, or abnormal blood counts detected on a complete blood count (CBC).

Abnormal Results

Positive MPO Expression: Strongly supports a diagnosis of myeloid lineage leukemia (AML). The percentage and intensity of MPO positivity help classify the leukemia subtype.

Negative MPO Expression: Suggests lymphoid lineage leukemia (ALL) or other non-myeloid disorders. However, results must be interpreted alongside morphology, cytogenetics, and additional markers.

Risks

The test requires a blood or bone marrow sample, typically collected through venipuncture or bone marrow aspiration. Risks are minimal but may include slight pain, bruising, or dizziness at the puncture site. Bone marrow aspiration may cause temporary soreness at the collection site.